Beyond imaging with coherent anti-Stokes Raman scattering microscopy

La microscopie optique permet de visualiser des échantillons biologiques avec une bonne sensibilité et une résolution spatiale élevée tout en interférant peu avec les échantillons. La microscopie par diffusion Raman cohérente (CARS) est une technique de microscopie non linéaire basée sur l’effet Raman qui a comme avantage de fournir un mécanisme de contraste endogène sensible aux vibrations moléculaires. La microscopie CARS est maintenant une modalité d’imagerie reconnue, en particulier pour les expériences in vivo, car elle élimine la nécessité d’utiliser des agents de contraste exogènes, et donc les problèmes liés à leur distribution, spécificité et caractère invasif. Cependant, il existe encore plusieurs obstacles à l’adoption à grande échelle de la microscopie CARS en biologie et en médecine : le coût et la complexité des systèmes actuels, les difficultés d’utilisation et d’entretient, la rigidité du mécanisme de contraste, la vitesse de syntonisation limitée et le faible nombre de méthodes d’analyse d’image adaptées. Cette thèse de doctorat vise à aller au-delà de certaines des limites actuelles de l’imagerie CARS dans l’espoir que cela encourage son adoption par un public plus large. Tout d’abord, nous avons introduit un nouveau système d’imagerie spectrale CARS ayant une vitesse de syntonisation de longueur d’onde beaucoup plus rapide que les autres techniques similaires. Ce système est basé sur un laser à fibre picoseconde synchronisé qui est à la fois robuste et portable. Il peut accéder à des lignes de vibration Raman sur une plage importante (2700–2950 cm-1) à des taux allant jusqu’à 10 000 points spectrales par seconde. Il est parfaitement adapté pour l’acquisition d’images spectrales dans les tissus épais. En second lieu, nous avons proposé une nouvelle méthode d’analyse d’images pour l’évaluation de la structure de la myéline dans des images de sections longitudinales de moelle épinière. Nous avons introduit un indicateur quantitatif sensible à l’organisation de la myéline et démontré comment il pourrait être utilisé pour étudier certaines pathologies. Enfin, nous avons développé une méthode automatisé pour la segmentation d’axones myélinisés dans des images CARS de coupes transversales de tissu nerveux. Cette méthode a été utilisée pour extraire des informations morphologique des fibres nerveuses dans des images CARS de grande échelle.Optical-based microscopy techniques can sample biological specimens using many contrast mechanisms providing good sensitivity and high spatial resolution while minimally interfering with the samples. Coherent anti-Stokes Raman scattering (CARS) microscopy is a nonlinear microscopy technique based on the Raman effect. It shares common characteristics of other optical microscopy modalities with the added benefit of providing an endogenous contrast mechanism sensitive to molecular vibrations. CARS is now recognized as a great imaging modality, especially for in vivo experiments since it eliminates the need for exogenous contrast agents, and hence problems related to the delivery, specificity, and invasiveness of those markers. However, there are still several obstacles preventing the wide-scale adoption of CARS in biology and medicine: cost and complexity of current systems as well as difficulty to operate and maintain them, lack of flexibility of the contrast mechanism, low tuning speed and finally, poor accessibility to adapted image analysis methods. This doctoral thesis strives to move beyond some of the current limitations of CARS imaging in the hope that it might encourage a wider adoption of CARS as a microscopy technique. First, we introduced a new CARS spectral imaging system with vibrational tuning speed many orders of magnitude faster than other narrowband techniques. The system presented in this original contribution is based on a synchronized picosecond fibre laser that is both robust and portable. It can access Raman lines over a significant portion of the highwavenumber region (2700–2950 cm-1) at rates of up to 10,000 spectral points per second and is perfectly suitable for the acquisition of CARS spectral images in thick tissue. Secondly, we proposed a new image analysis method for the assessment of myelin health in images of longitudinal sections of spinal cord. We introduced a metric sensitive to the organization/disorganization of the myelin structure and showed how it could be used to study pathologies such as multiple sclerosis. Finally, we have developped a fully automated segmentation method specifically designed for CARS images of transverse cross sections of nerve tissue.We used our method to extract nerve fibre morphology information from large scale CARS images

Chandra X-ray Observations of 12 Millisecond Pulsars in the Globular Cluster M28

We present a Chandra X-ray Observatory investigation of the millisecond pulsars (MSPs) in the globular cluster M28 (NGC 6626). In what is one of the deepest X-ray observations of a globular cluster, we firmly detect seven and possibly detect two of the twelve known M28 pulsars. With the exception of PSRs B1821-24 and J1824-2452H, the detected pulsars have relatively soft spectra, with X-ray luminosities 10^30-31 ergs s^-1 (0.3-8 keV),similar to most "recycled" pulsars in 47 Tucanae and the field of the Galaxy, implying thermal emission from the pulsar magnetic polar caps. We present the most detailed X-ray spectrum to date of the energetic PSR B1821-24. It is well described by a purely non-thermal spectrum with spectral photon index 1.23 and luminosity 1.4x10^33Theta(D/5.5 kpc)^2 ergs s^-1 (0.3-8 keV), where Theta is the fraction of the sky covered by the X-ray emission beam(s). We find no evidence for the previously reported line emission feature around 3.3 keV, most likely as a consequence of improvements in instrument calibration. The X-ray spectrum and pulse profile of PSR B1821--24 suggest that the bulk of unpulsed emission from this pulsar is not of thermal origin, and is likely due to low-level non-thermal magnetospheric radiation, an unresolved pulsar wind nebula, and/or small-angle scattering of the pulsed X-rays by interstellar dust grains. The peculiar binary PSR J1824-2452H shows a relatively hard X-ray spectrum and possible variability at the binary period, indicative of an intrabinary shock formed by interaction between the relativistic pulsar wind and matter from its non-degenerate companion star.Comment: 9 pages, 6 figures. Accepted for publication in the Astophysical Journa

F.A.R.O.G. FORUM, Vol. 5 No. 2

https://digitalcommons.library.umaine.edu/francoamericain_forum/1060/thumbnail.jp

Eight New Millisecond Pulsars in NGC 6440 and NGC 6441

Motivated by the recent discovery of 30 new millisecond pulsars in Terzan 5, made using the Green Bank Telescope's S-band receiver and the Pulsar Spigot spectrometer, we have set out to use the same observing system in a systematic search for pulsars in other globular clusters. Here we report on the discovery of five new pulsars in NGC 6440 and three in NGC 6441; each cluster previously had one known pulsar. Using the most recent distance estimates to these clusters, we conclude that there are as many potentially observable pulsars in NGC 6440 and NGC 6441 as in Terzan 5. We present timing solutions for all of the pulsars in these globular clusters. Four of the new discoveries are in binary systems; one of them, PSR J1748-2021B (NGC 6440B), has a wide (P_b = 20.5 d) and eccentric (e = 0.57) orbit. This allowed a measurement of its rate of advance of periastron: 0.00391(18) degrees per year. If due to the effects of general relativity, the total mass of this binary system is 2.92 +/- 0.20 solar masses (1 sigma), implying a median pulsar mass of 2.74 +/- 0.21 solar masses. There is a 1 % probability that the inclination is low enough that pulsar mass is below 2 solar masses, and 0.10 % probability that it is between 1.20 and 1.44 solar masses. If confirmed, this anomalously large mass would strongly constrain the equation of state for dense matter. The other highly eccentric binary, PSR J1750-37A, has e = 0.71, and periastron advance of 0.0055(3) degrees per year, implying a total system mass of 1.97 +/-0.15 solar masses and, along with the mass function, maximum and median pulsar masses of 1.65 and 1.26 solar masses respectively.Comment: Accepted for publication by the Astrophysical Journal. 14 pages in emulate format, 6 tables, 7 figure

F.A.R.O.G. FORUM, Vol. 5 No. 1

https://digitalcommons.library.umaine.edu/francoamericain_forum/1059/thumbnail.jp

Un engagement collectif pour maintenir et rehausser les compétences en littératie des adultes : avis à la ministre de l'éducation, du loisir et du sport et au ministre de l'enseignement supérieur, de la recherche de la science et de la technologie. /

Bibliogr. : p. 167-182

Un engagement collectif pour maintenir et rehausser les compétences en littératie des adultes. avis à la ministre de l'éducation, du loisir et du sport et au ministre de l'enseignement supérieur, de la recherche, de la science et de la technologie /

Comprend des références bibliographique